| Class XII |
Biotechnology |
Biotechnology |
2 |
two most common phages, whose genome has been frequently used to make cloning vectors for E. coli host. They are described in the following section... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
stranded DNA containing 48,490 base pairs. Each end has a 12 bases singlestrandedsegmentknownascossequences(cohesive ends). The two cohesive ends a... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
DNA (rDNA) lies between 38 to 52 kbp for its efficient packing into the λ head . Typical vectors coming out of lambda genome fall into two broad cl... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
(a) Bacteriophage M13 (b) Fig. 2.9: M13 bacteriophage (a) single stranded genome (b) M13 genome indicating different genes I to X a tube-like struc... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
2025-26 Conjugation pilus F plasmids that carry a DNA sequence encoding for the fertility factor, or F– Bacterial chromosome F plasmid factor. The ... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
Cosmid vector cos site contributed by plasmid DNA, combined ampR with a λ DNA segment that includes the joined cohesive ends (cos sites) (Fig. 2.11... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
the most common is the baker’s yeast, Saccharomyces cerevisiae. Although yeast can reproduce sexually, the cells usually multiply asexually by budd... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
sequence maker for sequence Selectable marker bacteria for yeast Fig. 2.13: Yeast Artificial Chromosome (YAC) Host-Vector System 21 Reprint 2025-26... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
promoter towards the 5' end (upstream) and a terminator at the 3' end (downstream) of the cloned gene in an expression vector. This portion of the ... |
|
| Class XII |
Computer Science |
Computer Science |
9 |
| 48000 | 695 | D02 | Salary,Bonus | 103 | Shabbir | 45000 | 566 | D01 | | 109 | Daribha | 42000 | NULL | D04 | DESC; | 105 | Joseph | 34000 | 875 ... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
host that provides various replication functions. • The cloning vector should be small in size and have an origin of replication (ori site), unique... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
• Expression vectors facilitate efficient-expression of cloned genes. They contain inducible promoters, unique insertion sites, and termination seq... |
|
| Class XII |
Biotechnology |
Biotechnology |
2 |
are false. 14. Assertion: Cosmid is a hybrid vector. Reason: Cosmid has properties of both plasmids and lambda phage vector. (a) Both assertion and... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
Chapter GeneCloning Gene cloning is a customary procedure to use a gene for its product in the biotechnology industry and various other 3.1 Identif... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
in the metabolic pathways (iron deficiency leading to chlorosis in plants) or resistance to environment (salinity tolerance in plants) or resistanc... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
acids, particularly makes it susceptible to cleavage when exposed to harsh physical stress. In addition, the chemical bonds by which different comp... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
Such mechanical or enzymatic treatment ruptures plasma membrane or cell wall so that nucleic acids get released from cells and exposes them to nucl... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
chelating agent ethylene diamine tetraacetic acid (EDTA). The mild alkaline pH facilitates the reduction of electrostatic interaction between DNA a... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
be separated by pipetting. The nucleic acids from aqueous phase are precipitated by addition of ethanol to aqueous medium that reduces its polarity... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
Flow chart for the isolation of RNA 32 BiotechnologyXII Reprint 2025-26 whereas chloroform solubilises lipids. Chloroform also enhances specific gr... |
|
| Class XII |
Chemistry |
Chemistry-II |
2 |
the acid strength. For this reason, the acid strength of alcohols decreases in the following order: 205 Alcohols, Phenols and Ethers Reprint 2026-2... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
ions (K ). The precipitate is then separated by centrifugation. The plasmid DNA is isolated from the supernatant by ethanol precipitation. Gene Clo... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
was awarded jointly to specific DNA sequence called recognition sequence Werner Arber, Daniel or site, often consisting of 4 to 8 bp. Nathans and H... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
strands of the DNA molecule within the restriction sequence recognised by the enzyme or at the site or near the sequence. It generates a 5′-phospha... |
|
| Class XII |
Biotechnology |
Biotechnology |
3 |
the microorganism. And the last Roman number represents the number of the enzyme isolated from the species (Table 3.2). BiotechnologyXII Reprint 20... |
|